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ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. In this case, with the pre-incubation aPTT mixing study result "corrected" and the post-incubation aPTT mixing study result "not corrected", a coagulation inhibitor should be suspected. Both anti-factor VIII and lupus anticoagulant have been known to be slow-acting and can exhibit this result pattern. If a factor deficiency was present, we should not see a post-incubation prolongation.
Hematology
What may cause the following mixing studies results?
Initial aPTT = 133 seconds
1:1 Mix aPTT pre-incubation = 33 seconds
1:1 Mix aPTT post-incubation = 124 seconds

A) Factor IX deficiency
B) A slow acting coagulation inhibitor
C) Factor XI deficiency

2. If all newborns were tested, many positive DATs due to ABO incompatibility would be detected that are of no clinical significance.
Although many laboratories test infants born to group O Rh positive females due to the higher risk of ABO HDFN when the mother is group O, testing such infants is optional provided there is appropriate monitoring and follow-up for hyperbilirubinemia.
Blood Bank
Not performing direct antiglobulin tests (DATs) on newborns born to group O Rh positive mothers is acceptable good practice, providing there is appropriate surveillance and follow-up to detect hyperbilirubinemia.

A) true
B) false

3. The Western Blot Assay is used as a confirmatory test for which of the following:

A) CMV Antibody
B) HBsAg
C) Anti-HCV
D) Anti-HIV-1

4. Escherichia coli and Klebsiella pneumoniae are two good quality control choices for the indole test. E. coli is indole positive, while K. pneumoniae is indole negative. A positive reaction is noted when there is a red layer at the top of the tube after the addition of Kovács reagent, while the negative result is a lack of color change in the top of the tube after the addition of Kovács reagent.
Which one of the following combinations of organisms would be appropriate positive and negative controls to verify the specific test functions listed?

A) Carbohydrate fermentation - Alcaligenes faecalis and Acinetobacter baumannii
B) Indole - Escherichia coli and Klebsiella pneumonia
C) Catalase - Staphylococcus aureus and Staphylococcus epidermidis
D) Beta hemolysis - Escherichia coli and Streptococcus pyogenes
E) Hydrogen sulfide production - proteus mirabilis and Salmonella typhi

5. What is the best description of the purpose of the Commission on Office Laboratory Accreditation (COLA) pertaining to the clinical laboratory?

A) CMS has given COLA deemed status to act on the government's behalf ot certify clinical laboratories
B) Administers both CLIA '88 and Medicare programs
C) Sets accreditation requirements for physician office laboratories (POLs)
D) Nonprofit educational group that establishes consensus standards for maintaining a high-quality laboratory organization

Solutions: